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KMID : 0613820060160061006
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Journal of Life Science
2006 Volume.16 No. 6 p.1006 ~ p.1013
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Application of Fluorescence Polarization Immunoassay for the Screening of Ochratoxin A in Unpolished Rice
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Park Jung-Hyun
Chung Duck-Hwa
Lee In-Seon
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Abstract
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To High Throughput Screening (HTS), a homogeneous fluorescence polarization immunoassay (FPIA) was developed for the quantitative determination of ochratoxin A(OTA) using a Victor³ (PerkinElmer). The homologous tracer, fluorescein-labelled OTA-EDF were synthesized and a specific OTA antibody has been used in the development of the method. It allowed the determination of OTA in the concentration range 0.5-200 ng/㎖, with the detection limit of 0.3 ng/㎖. The method developed was highly specific and reproducible. OTA spikes in unpolished rice extracts were determinable by FPIA with good recovery. For naturally contaminated unpolished rice samples some disagreement was observed between the results obtained by FPIA and HPLC, which could be related to the a little matrix effect observed for FPIA. Further research is needed to validate the procedure. On the basis of these initial results, this FPIA appears to meet the performance criteria for OTA screening of food samples without a complicated clean-up.
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KEYWORD
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Fluorescence polarization immunoassay, ochratoxin A, monoclonal antibody, unpolished rice
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